Trend of serum-free cryopreservative media
Abstract
Abstract
Cryopreservation is very important to stock precious tissues and cells including multipotent cells and primary cells without reducing their functions such as proliferation and differentiation. Traditional freezing media contain fetal bovine serum (FBS) for protecting cells and dimethyl sulfoxide (DMSO) as an anti-freezing reagent. However FBS has some risks such as pathogen contamination, high cost, variety of lot and shortage of supply, and DMSO will hurt cells in thawing process and after re-culture.In order to establish safer, more stable and valuable cryopreservation, serum-free freezing media has been proposed. Recombinant serum albumin, sericin and rakkyo fructan are prospective compounds as alternatives to FBS. Ethylene glycol (EG) and propylene glycol (PG) are used for serum-free freezing solutions as anti-freezing reagents combined with other cryopreservative compounds. There are 21 commercialized serum-free freezing media however most of their contents are not open. Therefore the confined information of research data will help youto find the optical serum-free freezing mediato various mammalian cells.
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DOI: http://dx.doi.org/10.18103/imr.v2i10.227
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